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adder skin needed for molecular analysis

Printed From: Reptiles and Amphibians of the UK
Category: Herpetofauna Native to the UK
Forum Name: Adder
Forum Description: Forum for all issues concerning Vipera berus
URL: http://www.herpetofauna.co.uk/forum/forum_posts.asp?TID=4944
Printed Date: 19 Jan 2021 at 5:26pm
Software Version: Web Wiz Forums 11.06 - http://www.webwizforums.com


Topic: adder skin needed for molecular analysis
Posted By: magdawu
Subject: adder skin needed for molecular analysis
Date Posted: 02 Jun 2015 at 11:35am
Dear All,

I am writing to you to ask if you maybe have a piece of dead adder skin found on the road or elsewhere. I am doing bioinformatic analysis of potential antimicrobial peptides in newly released Vipera berus DNA sequences - this is my MSc project. However, because I found some potential peptide sequences, I would like to try to extract it from adder's DNA and test it for its antimicrobial properties. I need just a small piece, up to several sq cm.

I would appreciate your help!

Magda



Replies:
Posted By: will
Date Posted: 03 Jun 2015 at 8:57am
just to clarify - you need actual dead adder fragment rather than sloughed skin?


Posted By: magdawu
Date Posted: 04 Jun 2015 at 1:50pm
Hi Will! Thank you for your reply! It actually can be both, sloughed skin should have enough DNA for the analysis I need. Do you have one?


Posted By: will
Date Posted: 04 Jun 2015 at 4:33pm
I'll have a look - just confirming that sloughs were OK.  I saw lots earlier in the season when the males were shedding, but I may be able to find some at my sites.


Posted By: Rags
Date Posted: 04 Jun 2015 at 5:45pm
Hi Magda, I think ARC have a stock pile of shed reptile skins. Drop them an e-mail.


Posted By: will
Date Posted: 04 Jun 2015 at 6:57pm
that's a very good point - when we were turning tins for smooth snakes a couple of weeks ago Ben Limburn from ARC was assiduously collecting any fragments of slowworm and smooth snake sloughs, and I guess they would do the same for adder sloughs as well!



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